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Figure 2 | Radiation Oncology

Figure 2

From: Live cell imaging at the Munich ion microbeam SNAKE – a status report

Figure 2

Z-projection reveals irradiation pattern. Hela cell stably transfected with a plasmid coding for a GFP- tagged version of the protein 53BP1 irradiated with 20 MeV protons (LET = 2.6 keV at cell position). 16 protons were applied per point in a 5 × 5 μm matrix pattern. Approximately 30 min after irradiation a series of images with defined z-distance through the whole nucleus was recorded (z-Stack). The images were deconvoluted to remove the blur caused by out-of- focus signals and a z-projection was performed. The radiation-induced 53BP1 foci are marked with arrows.

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