WTI-induced changes in the T cell phenotype are associated with altered surface expression of immunoregulatory molecules on CD4+ T cells. C57BL/6 mice received 0 Gy or 15 Gy whole thorax irradiation. At different time points, immune cells were isolated from lung tissue and stained for flow cytometric analysis. (A) Expression of CD39 on gated CD4+ T cells in the lung. (B) Expression of CD73 on gated CD4+ T cells in the lung. (C) Detection of CTLA-4 expression on gated CD4+ T cells in the lung. (D) Expression of CD103 on gated CD4+ T cells in the lung. Timelines of the indicated cell populations are shown as mean values ± SEM of percentages calculated on total lung cells. Cells of 4-6 mice per group were analyzed, * p ≤ 0.05; *** p ≤ 0.001, two-way ANOVA followed by post-hoc Bonferroni test.