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Table 1 DNA damage measured by the histone γ-H2AX in PBMCs isolated from blood of apparently healthy donors (N) and unselected breast cancer (BC) patients after exposure to 0.5 or 2 Gy of X-irradiation in vitro * or after 5 clinical fractions (BC patients)

From: Radiosensitivity in breast cancer assessed by the histone γ-H2AX and 53BP1 foci

Subjecta Age (years) Sex Clinical status with respect to cancer, skin reaction to RTb 0 Gy 0.5 Gy, 30’ 0.5 Gy, 24 h 2 Gy, 24 h 72 h after 5 clinical fractions
Apparently healthy donors
N-1 27 F n.d. 0.02 3.11   0.94  
N-2 28 F n.d. 0.04 2.60   0.86  
N-3 29 F n.d. 0.13 1.94 0.29 0.55  
N-4 58 F n.d. 0.07 2.00 0.28 0.41  
N-5 58 F n.d. 0.15 2.86 0.47 0.39  
N-6 65 F n.d. 0.12 2.73 0.42 0.74  
N-7 56 F n.d. 0.05 1.72 0.15 0.28  
N-8 48 F n.d. 0.41 2.67 0.28 0.60  
N-9 41 F n.d. 0.17 2.49 0.29 0.37  
N-10 44 F n.d. 0.07 2.09 0.19 0.34  
N-11 48 F n.d. 0.08 2.33 0.15 0.47  
N-12 43 F n.d. 0.10 2.23 0.19 0.36  
Mean 45    0.12 2.40 0.27 0.53  
± SD 12    0.10 0.40 0.10 0.21  
Breast cancer patients
BC-01 59 F BC, grade 3 0.70 3.29   2.59 0.32
BC-02 63 F BC, grade 1 0.62 2.39   2.93 0.57
BC-03 45 F BC, grade 0 0.92 2.87   3.93 0.67
BC-04 73 F BC, grade 3 0.42 4.50   2.61 0.66
BC-05 57 F BC, grade 1 0.07 5.48   2.50 0.49
BC-06 50 F BC, grade 1 0.27 4.77   1.72 0.56
BC-07 63 F BC, grade 2 0.46 5.32   3.65 0.75
BC-08 70 F BC, grade 2 0.84 5.49   4.47 0.64
BC-12 61 F BC, grade 3 0.25 4.71   2.26 0.57
BC-13 55 F BC, grade 3 0.32 5.34   3.11 0.83
BC-14 54 F BC, grade 2 0.14 3.75   1.18 0.19
BC-20 52 F BC, grade 1 0.32 3.77   1.95 1.06
BC-23 51 F BC, grade 1 0.12 2.67   2.25 0.77
BC-25 41 F BC, grade 0 0.44 4.99   1.27 0.30
BC-26 63 F BC, grade 1 0.21 3.62   1.92 0.35
BC-27 71 F BC, grade 1 0.14 3.31   0.92 0.57
BC-28 42 F BC, grade 0 0.10 4.29   1.29 0.35
BC-29 74 F BC, grade 1 0.22 3.7   1.12 0.28
BC-30 46 F BC, grade 2 0.05 3.39   0.77 0.32
BC-31 56 F BC, grade 2 0.01 1.95   0.95 0.27
BC-32 44 F BC, grade 2 0.08 2.98   1.08 0.28
BC-33 41 F BC, grade 3 0.19 3.43   0.92 0.29
BC-34 38 F BC, grade 2 0.10 3.22   0.86 0.28
BC-35 77 F BC, grade 2 0.07 3.23   0.91 0.06
BC-36 70 F BC, grade 1 0.06 2.72   0.81 0.18
BC-37 64 F BC, grade 0 0.03 2.90   0.48 0.19
BC-38 73 F BC, grade 1 0.08 2.37   0.48 0.20
BC-39 55 F BC, grade 1 0.01 2.05 0.13 0.31 0.43
BC-40 77 F BC, grade 2 0.05 2.24 0.11 0.15 0.34
BC-41 54 F BC, grade 1 0.09 2.11 0.31 0.39 0.38
BC-42 49 F BC, grade 1 0.01 2.05 0.15 0.40 0.34
BC-43 62 F BC, grade 2 0.05 1.99 0.43 0.62 0.57
BC-44 59 F BC, grade 1 0.04 2.54 0.41 0.75 0.18
BC-45 51 F BC, grade 1 0.02 2.81 0.40 0.64 0.24
BC-46 62 F BC, grade 1 0.32 3.58 0.70 0.28 0.32
BC-47 30 F BC, grade 2 0.22 3.27 0.41 0.24 0.18
BC-48 71 F BC, grade 2 0.26 3.48 0.46 0.59 0.51
BC-49 46 F BC, grade 2 0.18 3.15 0.38 0.60 0.30
BC-50 48 F BC, grade 3 0.13 3.34 0.33 0.31 0.34
BC-51 57 F BC, grade 1 0.09 2.63 0.39 0.34 0.41
BC-52 62 F BC, grade 2 0.09 2.54 0.25 0.36 0.31
BC053 68 F BC, grade 2 0.09 2.23 0.20 0.34 0.16
BC-54 37 F BC, grade 1 0.14 2.28 0.25 0.47 0.4
BC-55 67 F BC, grade 1 0.08 2.73 0.41 0.4 0.27
BC-57 55 F BC, grade 1 0.11 2.68 0.3 0.76 0.38
BC-58 62 F BC, grade 1 0.06 2.62 0.31 0.6 0.32
BC-59 55 F BC, grade 1 0.07 1.85 0.22 0.41 0.36
BC-60 65 F BC, grade 2 0.11 3.32 0.42 0.54 0.43
BC-61 54 F BC, grade 2 0.04 2.09 0.25 0.52 0.43
BC-62 53 F BC, grade 2 0.06 0.06 0.19 0.14 0.38
BC-63 69 F BC, grade 1 0.09 2.65 0.34 0.59 0.4
BC-64 81 F BC, grade 1 0.1 2.65 0.39 0.36 0.27
BC-65 38 F BC, grade 2 0.15 2.01 0.31 0.67 0.31
BC-66 77 F BC, grade 1 0.14 2.29 0.3 0.64 0.27
BC-67 48 F BC, grade 1 0.14 2.61 0.45 0.71 0.36
BC-68 49 F BC, grade 2 0.1 2.48 0.22 0.53 0.27
BC-69 51 F BC, grade 1 0.26 2.25 0.2 0.53 0.16
Mean 57    0.18 3.07 0.32 1.11 0.39
± SD 12    0.19 1.04 0.12 1.01 0.19
  1. *Each indicated DNA damage parameter represents the mean value obtained on the cells from a given individual. Bold fonts indicate the unselected BC patients who revealed an adverse acute skin reaction to RT;
  2. aCase number was according to our files;
  3. bEarly skin reaction according RTOG score (Cox et al., 1995) [26]. RTOG grade: 1 - follicular, faint or dull erythema, dry desquamation; 2 - tender or bright erythema, moderate edema; 3 - confluent, moist desquamation, pitting edema; 4 - ulceration, haemorrhage, necrosis.
  4. Abbreviations used: N, normal; BC, breast cancer; F, female; n.d., not determined.