Comparing treatment outcomes of different chemotherapy sequences during intensity modulated radiotherapy for advanced N-stage nasopharyngeal carcinoma patients
- Xueming Sun†1, 2,
- Lei Zeng†1, 2,
- Chunyan Chen1, 2,
- Ying Huang1, 2,
- Fei Han1, 2,
- Weiwei Xiao1, 2,
- Shuai Liu1, 2 and
- Taixiang Lu1, 2Email author
© Sun et al.; licensee BioMed Central Ltd. 2013
Received: 20 April 2013
Accepted: 20 October 2013
Published: 13 November 2013
N-stage is related to distant metastasis of nasopharyngeal carcinoma (NPC) patients. We performed this study to compare the efficacy of different chemotherapy sequences in advanced N-stage (N2 and N3) NPC patients treated with intensity modulated radiotherapy (IMRT).
From 2001 to 2008, 198 advanced N-stage NPC patients were retrospectively analyzed. Thirty-three patients received IMRT alone. Concurrent chemoradiotherapy (CCRT) was delivered to 72 patients, neoadjuvant chemotherapy (NACT) + CCRT to 82 patients and CCRT + adjuvant chemotherapy (AC) to 11 patients.
The 5-year overall survival rate, recurrence-free survival rate, distant metastasis-free survival rate and progress-free survival rate were 47.7% and 73.1%(p<0.001), 74.5% and 91.3% (p = 0.004), 49.2% and 68.5% (p = 0.018), 37.5% and 63.8% (p<0.001) in IMRT alone and chemoradiotherapy group. Subgroup analyses indicated that there were no significant differences among the survival curves of CCRT, NACT + CCRT and CCRT + AC groups. The survival benefit mainly came from CCRT. However, there was only an improvement attendency in distant metastasis-free survival rate of CCRT group (p = 0.107) when compared with RT alone group, and NACT + CCRT could significantly improve distant metastasis-free survival (p = 0.017).
For advanced N-stage NPC patients, NACT + CCRT might be a reasonable treatment strategy.
Although rare among the Caucasian population, nasopharyngeal carcinoma (NPC) is rather common among Asians, especially the Southern Chinese . Intensity-modulated radiotherapy (IMRT) is a major breakthrough in the treatment of NPC. It enables the delivery of higher radiation dose to the lesion while sparing the organs/tissues at risk, thus enhancing the therapeutic ratio, and has been accepted as a more advantageous technique as compared with two-dimensional conventional radiotherapy (2D-CRT). Previous studies have confirmed that IMRT changed the failure pattern of NPC from local recurrence and distant metastasis to predominantly distant metastasis in patients treated with 2D-CRT [2, 3].
It is well known that N-stage is related to distant metastasis of NPC patients. Wong et al.  reviewed 175 NPC patients treated with IMRT and found that the 3-year distant failure-free survival rate was 95.4%, 100%, 76.6%, and 67.3% for stage N0, N1, N2, and N3 patients, respectively. The Cox proportional hazards regression analysis showed that advanced N-stage was a significant predictor of distant metastasis (p = 0.029). Therefore, it is necessary to explore more effective combined chemoradiation strategies to improve the outcomes of advanced N-stage patients with NPC.
According to the results of previously reported phase III clinical trial and meta-analysis [4–6], concurrent chemoradiotherapy had the most definite survival benefit for locoregionally advanced NPC and had became a standard treatment regimen. However, in these trials and meta-analysis, 2D-CRT technique was adopted for radiotherapy. To our knowledge,there was no phase β clinical trials to identify the value of chemotherapy on locoregionally advanced NPC treated with IMRT. Especially for advanced N-stage patients (whose key problem is distant failure), it is unknown whether chemotherapy could reduce distant metastasis rate and improve treatment outcomes. Therefore, we retrospectively analyzed the long term outcomes of advanced N-stage NPC patients treated with IMRT, and performed the analysis to compare the efficacy of different chemotherapy sequences in these patients.
Patients and patient workup
Baseline characteristics of the 198 patients
NACT + CCRT
CCRT + AC
Target volume delineation and evaluation of IMRT planning
All patients were immobilized in the supine position with a head, neck, and shoulder thermoplastic mask. CT simulation was performed with a slice thickness of 3 mm extending from the vertex to 2 cm below the clavicle. Both plain CT and contrast-enhanced CT images were obtained and transferred to inverse treatment-planning system (CORVUS 3.0/3.2,Peacock plan) developed by NOMOS Corporation. The primary tumor area and the upper-neck area above the caudal edge of the cricoids cartilage were treated by IMRT. Target volumes were delineated according to our institutional treatment protocol , in agreement with the International Commission on Radiation Units and Measurements Reports (ICRU)50 and 62. Planning target volumes (PTVs) for all gross tumor volumes and clinical target volumes (CTVs) were generated automatically after delineation of tumor targets according to the immobilization and localization uncertainties.
Inverse planning was performed on the Corvus System for all patients using Simultaneous Modulated Accelerated Radiation Therapy boost RT . The prescribed dose was 68Gy to the PTV of the GTVnx, 60Gy to the PTV of CTV1, 54Gy to the PTV of CTV2, and 60-66Gy to the PTV of the GTV for the positive cervical lymph nodes in 30 fractions. The prescribed dose to the lower neck and the supraclavicular fossae by irradiation with conventional RT technique was 50Gy/25 fraction for prophylactic intent in whole neck group.
The dose-volume histograms of the treatment targets and critical normal structures were evaluated. For GTV and CTV, the target volumes receiving ≥ 95% of the prescribed dose was used to reflect the target coverage. For the critical organs with functional subunits organized in series, the dose to 5% of the volume couldn’t exceed their tolerance doses. For the critical organs with functional subunits organized in parallel, the dose constraints to 33% of the volume were less than their tolerance doses.
During the study period, we followed our institutional guidelines (Sun Yat-sen University Cancer Center IRB), which recommend both neoadjuvant or adjuvant chemotherapy and/or concomitant chemotherapy for the patients. Deviation from the institutional guidelines were because of organ dysfunction, suggesting intolerance to the chemotherapy, and patient refusal.
Chemotherapy was administered to 165 patients. Concurrent chemotherapy (CCT) was delivered to 72 patients, neoadjuvant chemotherapy (NACT) + CCT to 82 patients and CCT + adjuvant chemotherapy (AC) to 11 patients. The regimens of NACT included TC (paclitaxel 135 mg/m2 IV on day 1 and carboplatin AUC = 6 IV on day 1) and PF (cisplatin 80 mg/m2 IV on day 1 and 5-Fu 800 mg/m2/d continuously IV on day 1–5). There were 33 patients received TC regimen and 49 patients received PF regimen. Regimens were repeated every 3 weeks for 2–3 cycles. CCT included three regimens. Cisplatin (CDDP) 80 mg/m2 IV every 3 weeks was delivered to 92 patients, CDDP 30-40 mg/m2 IV weekly was delivered to 47 patients, and PF regimen (the same as NACT) was delivered to 26 patients. For patients who received adjuvant chemotherapy, PF regimen (the same as NACT) were repeated every 3 weeks for 2–4 cycles.
Follow-up and statistical analysis
After RT completion, the patients were subsequently followed up monthly for the first 3 months, every 3 months through 3 years, every 6 months for the next 2 years, and then annually. The median follow-up period was 56 months (range, 3–120 months). The survival time was measure from first day of RT completion to the date of the event or last follow-up visit. The Statistical Package for Social Sciences, version 16.0 (SPSS,Chicago, IL) was used for statistical analysis. The Kaplan-Meier method was used to calculate the overall survival (OS), recurrence-free survival (RFS), distant metastasis-free survival (DMFS), and progress-free survival (PFS) rates. The log-rank test was used to compare the survival curves. All statistical tests were two-sided, and p < 0.05 was considered statistically significant.
Patterns of failure in the 198 patients after treatment
Patterns of failure
NACT + CCRT
CCRT + AC
Primary, nodal recurrence
Distant metastasis, Primary and/or nodal recurrence
Univariate analysis for various clinical endpoints
Multivariate analysis for various clinical endpoints
Treatment-related toxicity was scored according to the Radiation Therapy Oncology Group radiation morbidity scoring criteria. For the patients treated with IMRT alone, the most frequently observed acute toxicity was mainly grade 1 or 2 xerostomia and mucositis. The incidence of acute grade 3 mucositis was 24.2%. Six patients (18.2%) had grade 1 leukopenia and/or neutropenia. No grade 4 acute toxicities were observed. For the patients treated with chemoradiotherapy, seventy-six patients (46.1%) had acute grade 3 mucositis and 34 patients (20.6%) had grade 3 vomiting. Seventeen patients developed grade 3 and 3 patients developed grade 4 neutropenia. And 9 patients had grade 3 or 4 thrombocytopenia. The most common severe acute hematologic toxicities (grade ≥ 3) were leukopenia and/or neutropenia n = 20 (30.8%) and thrombocytopenia n = 9 (5.5%).
The Frequency of late toxicities for the patients after treatment
IMRT alone (n = 26)
Chemoraditherapy (n = 126)
Grade 1 no.(%)
Grade 2 no.(%)
Grade 3 no.(%)
Grade 1 no.(%)
Grade 2 no.(%)
Grade 3 no.(%)
Temporal lobe injury
Summary of the Studies Reporting DMFS and OS of advanced N-stage NPC patients
Technique of RT
Leung, et al 
Ma, et al 
Lai, et al 
Wong, et al 
Yi, et al 
Intergroup study 0099  demonstrated that CCRT + AC is superior to radiotherapy alone for locoregionally advanced NPC patients with respect to PFS and OS. However, the phase III randomized study in endemic regions did not achieve similar results . Recently, a comparison between concurrent chemoradiotherapy followed by adjuvant chemotherapy and concurrent chemoradiotherapy was performed in 508 locally advanced NPC patients enrolled in a phase III trial conducted by Chen et al. . In this trial, concurrent chemoradiotherapy followed by adjuvant chemotherapy did not significantly improve PFS. The meta-analysis by Baujat et al.  indicated that the OS benefit is 6% with the use of chemotherapy, and the benefit is largely owing to the CCRT. No evidence of prognostic benefit was observed with adjuvant chemotherapy. The other meta-analysis  also demonstrated that CCRT was the most effective treatment modality for the improvement of survival. However, most patients in the former studies were treated with 2D-CRT. But IMRT could increase the dose conformity and irradiate higher dose to the treatment targets, so the locoregional control was improved significantly [15, 16]. Therefore, the margin of benefit potentially gained with additional chemotherapy maybe reduced. Lin et al.  have questioned the role of concurrent chemotherapy in advanced NPC patients treated with IMRT. In the present study, compared with IMRT alone, CCRT could significantly improve OS, RFS, PFS of advanced N-stage patients. The possible reason is most advanced N-stage patients also with advanced T-stage. Hara et al.  found that although treated with IMRT, the local failure rate was still more than 10% in stage T3/4 NPC patients. Larger tumors are related to adverse radiobiological parameters, including an increased clonogen number [19, 20] and hypoxia[21, 22], which are difficult to overcome with dose escalation alone. In this case, concurrent chemotherapy could still enhance the radiosensitivity to improve locoregional control. And we also observed that compared with IMRT alone, there was only a trend toward a lower distant failure rate with CCRT, but the correlation did not reach statistical significance (p = 0.107). CCRT alone is ineffective in reducing distant metastasis maybe is a reasonable explanation . Moreover, we found even though CCRT + AC group have higher OS, RFS and PFS than IMRT alone group, the differences of survival rates between CCRT + AC and CCRT group did not reach statistical significance. So maybe the survival benefit of CCRT + AC is largely owing to the CCRT. Adjuvant chemotherapy did not improve prognosis yet. But although the survival rates of CCRT + AC group did not reach statistical significance when compared with CCRT or NACT + CCRT group, we observed this group had the highest survival rates in the three groups. This phenomenon maybe explained by the small number of patients treated with CCRT + AC, and the same N2 stage of all the 11 patients.
The role of neoadjuvant chemotherapy followed by concurrent chemoradiotherapy or RT is a matter of outstanding interest. The pool data analysis by Chua et al.  found NACT + RT arm was associated with a significant improvement in locoregional relapse-free survival and disease-specific survival in advanced-stage NPC, but not in OS. Hui et al.  published the results of a randomized phase II trial in which stage III-IVb NPC patients were randomly assigned to receive either NACT + CCRT, or CCRT alone. A positive impact on survival was observed, since the 3-year overall survival for the NACT + CCRT versus the CCRT arm was 94.1% vs.67.7% (p = 0.012).The present study demonstrated that the differences of survival rates between NACT + CCRT and CCRT groups did not reach statistical significance. So the survival benefit of CCRT + AC maybe is mainly owing to the CCRT. However, compared with IMRT alone, NACT + CCRT could improve DMFS rate significantly, while there was only a trend with CCRT. Maybe in the condition of such a high locoregional control by CCRT, NACT + CCRT is unlikely to improve RFS rate significantly. But because of more powerful than concurrent chemoradiotherapy, NACT might be effective in controlling micrometastatic dissemination.
In this study, we have described the long-term outcomes for patients with advanced N-stage NPC who underwent IMRT with the SMART boost technique. Compared with IMRT alone, CCRT could significantly improve OS, RFS, PFS of advanced N-stage patients, and NACT + CCRT could significantly improve DMFS. There was no evidence of survival benefit was observed with adjuvant chemotherapy in this study. So, according to the results of the present study, NACT + CCRT might be a reasonable strategy for advanced N-stage NPC patients. However, our study was limited by the small sample size, use of retrospective analysis, and possible selection bias during the matching process. As a result, relative randomized clinical trial is still needed in the future. In addition, although an excellent locoregional control was achieved by IMRT, the DMFS and OS were not improved compared with the advanced N-stage patients undergoing 2D-CRT. New strategies combining different treatment modalities to reduce the rate of distant metastasis effectively also need to be developed in the future.
- Yu MC: Diet and nasopharyngeal carcinoma. Prog Clin Biol Res. 1990, 346: 93-PubMedGoogle Scholar
- Wong FC, Ng AW, Lee VH, Lui CM, Yuen KK, Sze WK, Leung TW, Tung SY: Whole-field simultaneous integrated-boost intensity-modulated radiotherapy for patients with nasopharyngeal carcinoma. Int J Radiat Oncol Biol Phys. 2010, 76 (1): 138-145. 10.1016/j.ijrobp.2009.01.084.View ArticlePubMedGoogle Scholar
- Lee N, Harris J, Garden AS, Straube W, Glisson B, Xia P, Bosch W, Morrison WH, Quivey J, Thorstad W, Jones C, Ang KK: Intensity-Modulated Radiation Therapy With or Without Chemotherapy for Nasopharyngeal Carcinoma: Radiation Therapy Oncology Group Phase II Trial 0225. J Clin Oncol. 2009, 27 (22): 3684-3690. 10.1200/JCO.2008.19.9109.View ArticlePubMedPubMed CentralGoogle Scholar
- Al-Sarraf M, LeBlanc M, Giri PG, Fu KK, Cooper J, Vuong T, Forastiere AA, Adams G, Sakr WA, Schuller DE, Ensley JF: Chemoradiotherapy versus radiotherapy in patients with advanced nasopharyngeal cancer: phase III randomized Intergroup study 0099. J Clin Oncol. 1998, 16 (4): 1310-1317.PubMedGoogle Scholar
- Baujat B, Audry H, Bourhis J, Chan AT, Onat H, Chua DT, Kwong DL, Al-Sarraf M, Chi KH, Hareyama M, Leung SF, Thephamongkhol K, Pignon JP: Chemotherapy in locally advanced nasopharyngeal carcinoma:An individual patient data meta-analysis of eight randomized trials and 1753 patients. Int J Radiat Oncol Biol Phys. 2006, 64 (1): 47-56. 10.1016/j.ijrobp.2005.06.037.View ArticlePubMedGoogle Scholar
- Zhang L, Zhao C, Ghimire B, Hong MH, Liu Q, Zhang Y, Guo Y, Huang YJ, Guan ZZ: The role of concurrent chemoradiotherapy in the treatment of locoregionally advanced nasopharyngeal carcinoma among endemic population: a meta-analysis of the phase III randomized trials. BMC Cancer. 2010, 15: 10-558.Google Scholar
- Zhao C, Lu TX, Han F, Lu LX, Huang SM, Deng XW, Lin CG, Cui NJ: Clinical study of 139 nasopharyngeal carcinoma patients for intensity modulated radiation therapy. Chin J Radiat Oncol. 2006, 1: 1-6. 10.1186/1748-717X-1-1.View ArticleGoogle Scholar
- Butler EB, Teh BS, Grant WH, Uhl BM, Kuppersmith RB, Chiu JK, Donovan DT, Woo SY: Smart (simultaneous modulated accelerated radiation therapy) boost: a new accelerated fraetionation schedule for the treatment of head and neck cancer with intensity modulated radiotherapy. Int J Radiat Oncol Biol Phys. 1999, 45: 21-32. 10.1016/S0360-3016(99)00101-7.View ArticlePubMedGoogle Scholar
- Leung TW, Tung SY, Sze WK, Wong FC, Yuen KK, Lui CM, Lo SH, Ng TY OSK: Treatment results of 1070 patients with nasopharyngeal carcinoma: an analysis of survival and failure patterns. Head Neck. 2005, 27 (7): 555-565. 10.1002/hed.20189.View ArticlePubMedGoogle Scholar
- Ma J, Mai HQ, Hong MH, Cui NJ, Lu TX, Lu LX, Mo HY, Min HQ: Is the 1997 AJCC staging system for nasopharyngeal carcinoma prognostically useful for Chinese patient populations?. Int J Radiat Oncol Biol Phys. 2001, 1;50 (5): 1181-1189.View ArticleGoogle Scholar
- Lai SZ, Li WF, Chen L, Luo W, Chen YY, Liu LZ, Sun Y, Lin AH, Liu MZ, Ma J: How does intensity-modulated radiotherapy versus conventional two-dimensional radiotherapy influence the treatment results in nasopharyngeal carcinoma patients?. Int J Radiat Oncol Biol Phys. 2011, 80 (3): 661-668. 10.1016/j.ijrobp.2010.03.024.View ArticlePubMedGoogle Scholar
- Yi JL, Gao L, Xu GZ, Huang XD, Luo JW, Li SY, Xiao JP, Cai LM: Treatment resulls of Intensity modulated radiotherapy for nasopharyngeal carcinoma: an analysis of 147 patients. Chin J Radiat Oncol. 2008, 17 (5): 329-334.Google Scholar
- Lee AW, Lau WH, Tung SY, Chua DT, Chappell R, Xu L, Siu L, Sze WM, Leung TW, Sham JS, Ngan RK, Law SC, Yau TK, Au JS, O’Sullivan B, Pang ES, SK O, Au GK, Lau JT: Preliminary results of a randomized study on therapeutic gain by concurrent chemotherapy for regionally-advanced nasopharyngeal carcinoma:NPC-9901 trial by the HongKong nasopharyngeal cancer study group. J Clin Oncol. 2005, 23 (28): 6966-6975. 10.1200/JCO.2004.00.7542.View ArticlePubMedGoogle Scholar
- Chen L, Hu CS, Chen XZ, Hu GQ, Cheng ZB, Sun Y, Li WX, Chen YY, Xie FY, Liang SB, Chen Y, Xu TT, Li B, Long GX, Wang SY, Zheng BM, Guo Y, Sun Y, Mao YP, Tang LL, Chen YM, Liu MZ, Ma J: Concurrent chemoradiotherapy plus adjuvant chemotherapy versus concurrent chemoradiotherapy alone in patients with locoregionally advanced nasopharyngeal carcinoma:a phase 3 multicentre randomized controlled trial. Lancet Oncol. 2012, 13 (2): 163-171. 10.1016/S1470-2045(11)70320-5.View ArticlePubMedGoogle Scholar
- Chau RM, Teo PM, Kam MK, Leung SF, Cheung KY, Chan AT: Dosimetric comparison between 2-dimensional radiation therapy and intensity modulated radiation therapy in treatment of advanced T-stage nasopharyngeal carcinoma: to treat less or more in the planning organ-at-risk volume of the brainstem and spinal cord. Med Dosim. 2007, 32 (4): 263-270. 10.1016/j.meddos.2007.02.006.View ArticlePubMedGoogle Scholar
- Kam MK, Chau RM, Suen J, Choi PH, Teo PM: Intensity-modulated radiotherapy in nasopharyngeal carcinoma: dosimetric advantage over conventional plans and feasibility of dose escalation. Int J Radiat Oncol Biol Phys. 2003, 56 (1): 145-157. 10.1016/S0360-3016(03)00075-0.View ArticlePubMedGoogle Scholar
- Lin S, Lu JJ, Han L, Chen Q, Pan J: Sequential chemotherapy and intensity-modulated radiation therapy in the management of locoregionally advanced nasopharyngeal carcinoma: experience of 370 consecutive cases. BMC Cancer. 2010, 10: 10-39. 10.1186/1471-2407-10-10.View ArticleGoogle Scholar
- Hara W, Loo BW, Goffinet DR, Chang SD, Adler JR, Pinto HA, Fee WE, Kaplan MJ, Fischbein NJ, Le QT: Excellent local control with stereotactic radiotherapy boost after external beam radiotherapy in patients with nasopharyngeal carcinoma. Int J Radiat Oncol Biol Phys. 2008, 71: 393-400. 10.1016/j.ijrobp.2007.10.027.View ArticlePubMedGoogle Scholar
- Withers HR, Taylor JM, Maciejewski B: The hazard of accelerated tumor clonogen repopulation during radiotherapy. Acta Oncol. 1988, 272: 131-146.View ArticleGoogle Scholar
- Johnson CR, Thames HD, Huang DT, Schmidt-Ullrich RK: The tumor volume and clonogen number relationship: Tumor control predictions based upon tumor volume estimates derived from computed tomography. Int J Radiat Oncol Biol Phys. 1995, 332: 281-287.View ArticleGoogle Scholar
- Lartigau E, Le Ridant AM, Lambin P, Weeger P, Martin L, Sigal R, Lusinchi A, Luboinski B, Eschwege F, Guichard M: Oxygenation of head and neck tumors. Cancer. 1993, 717: 2319-2325.View ArticleGoogle Scholar
- Okunieff P, Hoeckel M, Dunphy EP, Schlenger K, Knoop C, Vaupel P: Oxygentension distributions are sufficient to explain the local response of human breast tumors treated with radiation alone. Int J Radiat Oncol Biol Phys. 1993, 26: 631-636. 10.1016/0360-3016(93)90280-9.View ArticlePubMedGoogle Scholar
- Chua DT, Sham JS, Au GK, Choy D: Concomitant chemoirradiation for stage III-IV nasopharyngeal carcinoma in Chinese patients: results of a matched cohort analysis. Int J Radiat Oncol Biol Phys. 2002, 53 (2): 334-343. 10.1016/S0360-3016(02)02710-4.View ArticlePubMedGoogle Scholar
- Chua DT, Ma J, Sham JS, Mai HQ, Choy DT, Hong MH, Lu TX, Min HQ: Long-term survival after cisplatin-based induction chemotherapy and radiotherapy for nasopharyngeal carcinoma: a pooled data analysis of two phase III trials. J Clin Oncol. 2005, 20;23 (6): 1118-1124.View ArticleGoogle Scholar
- Hui EP, Ma BB, Leung SF, King AD, Mo F, Kam MK, Yu BK, Chiu SK, Kwan WH, Ho R, Chan I, Ahuja AT, Zee BC, Chan AT: Randomized phase II trial of concurrent cisplatin-radiotherapy with or without neoadjuvant docetaxel and cisplatin in advanced nasopharyngeal carcinoma. J Clin Oncol. 2009, 27: 242-249. 10.1200/JCO.2008.18.1545.View ArticlePubMedGoogle Scholar
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