TIMP-1 and TIMP-2 protein levels are regulated by IR and patupilone. HT1080 cells were treated with patupilone (0.2 nM) and IR (2, 10 Gy) or PMA (0.65 mM). A, TIMP mRNA levels were determined using qRT-PCR, n > 4. B and C, protein levels of TIMPs were determined by western blotting in the CM (B) or cell lysate (C). Representative blots are shown; quantification was performed with at least 4 independent experiments. * indicates a TIMP-1 band, which was still detectable after washing and restaining of the membrane with TIMP-2 antibody. *P < 0.05, **P < 0.01.