- Open Access
Technology assessment of automated atlas based segmentation in prostate bed contouring
© Hwee et al; licensee BioMed Central Ltd. 2011
Received: 10 June 2011
Accepted: 9 September 2011
Published: 9 September 2011
Prostate bed (PB) contouring is time consuming and associated with inter-observer variability. We evaluated an automated atlas-based segmentation (AABS) engine in its potential to reduce contouring time and inter-observer variability.
An atlas builder (AB) manually contoured the prostate bed, rectum, left femoral head (LFH), right femoral head (RFH), bladder, and penile bulb of 75 post-prostatectomy cases to create an atlas according to the recent RTOG guidelines. 5 other Radiation Oncologists (RO) and the AABS contoured 5 new cases. A STAPLE contour for each of the 5 patients was generated. All contours were anonymized and sent back to the 5 RO to be edited as clinically necessary. All contouring times were recorded. The dice similarity coefficient (DSC) was used to evaluate the unedited- and edited- AABS and inter-observer variability among the RO. Descriptive statistics, paired t-tests and a Pearson correlation were performed. ANOVA analysis using logit transformations of DSC values was calculated to assess inter-observer variability.
The mean time for manual contours and AABS was 17.5- and 14.1 minutes respectively (p = 0.003). The DSC results (mean, SD) for the comparison of the unedited-AABS versus STAPLE contours for the PB (0.48, 0.17), bladder (0.67, 0.19), LFH (0.92, 0.01), RFH (0.92, 0.01), penile bulb (0.33, 0.25) and rectum (0.59, 0.11). The DSC results (mean, SD) for the comparison of the edited-AABS versus STAPLE contours for the PB (0.67, 0.19), bladder (0.88, 0.13), LFH (0.93, 0.01), RFH (0.92, 0.01), penile bulb (0.54, 0.21) and rectum (0.78, 0.12). The DSC results (mean, SD) for the comparison of the edited-AABS versus the expert panel for the PB (0.47, 0.16), bladder (0.67, 0.18), LFH (0.83, 0.18), RFH (0.83, 0.17), penile bulb (0.31, 0.23) and rectum (0.58, 0.09). The DSC results (mean, SD) for the comparison of the STAPLE contours and the 5 RO are PB (0.78, 0.15), bladder (0.96, 0.02), left femoral head (0.87, 0.19), right femoral head (0.87, 0.19), penile bulb (0.70, 0.17) and the rectum (0.89, 0.06). The ANOVA analysis suggests inter-observer variability among at least one of the 5 RO (p value = 0.002).
The AABS tool results in a time savings, and when used to generate auto-contours for the femoral heads, bladder and rectum had superior to good spatial overlap. However, the generated auto-contours for the prostate bed and penile bulb need improvement.
Radiotherapy as an adjunct to radical prostatectomy for prostate cancer with adverse features such as pT3 and margin positive disease has established benefits of reduced disease recurrence and improved clinical outcomes . Increasingly, prostate bed radiotherapy is being delivered with intensity modulated radiotherapy (IMRT) and/or image-guided radiotherapy (IGRT) which have both facilitated dose escalation to target tissues while sparing adjacent normal structures. This has improved the therapeutic ratio. However, these advanced technologies require the radiation oncologist to have a comprehensive understanding of cross sectional anatomy as compared to conventionally planned treatment (based on skeletal landmarks) for the accurate delineation and dose coverage of target volumes and organs at risk (OARs) . Inadequate coverage of the prostate bed has been demonstrated to lead to an increased risk of local recurrence .
Significant levels of inter- and intra-observer variability in target volume delineation (TVD) has been repeatedly demonstrated in prostate cancer radiotherapy [4–7]. In fact, it has been argued that inter-observer TVD variability is the most significant contributor to uncertainty in radiation treatment planning . A recent development in Radiation Oncology is the use of automated atlas-based segmentation (AABS) algorithms to aid in TVD. AABS is a computer-assisted tool that utilizes an algorithm that resamples local data to automatically outline the structures of interest to be irradiated. AABS algorithms have the potential to address the variability and time-intensive problems associated with manual contouring.
As with most technologies that are rapidly being introduced into Radiation Oncology practice, the evaluation of AABS in the form of traditional clinical trials can be costly and is likely unfeasible . The purpose of this paper is to evaluate the accuracy, reliability and potential time-savings of an AABS. Secondly, we assessed inter- and intra-observer variability in the delineation of the post-prostatectomy clinical target volume (CTV) (prostate bed) and relevant organs at risk (OARs).
Eighty post-prostatectomy patients planned for adjuvant or salvage radiotherapy from January to December 2009 were randomly selected as part of this University of Western Ontario Research Ethics Board approved study. All patients were scanned in the supine position, from L4 to the ischial tuberosities. The computed tomography (CT) images were saved according to the Digital Imaging and Communications in Medicine (DICOM) standards of practice. For all three stages of this protocol, physicians were asked to contour the prostate bed and OARs (bladder, rectum, penile bulb, bilateral femora) according to the recently published Radiation Therapy Oncology Group (RTOG) guidelines for post-prostatectomy radiotherapy .
The atlas builder (GR) manually contoured an index case and inserted the contoured CT image into the atlas. A second patient was randomly selected to have the MIM atlas-based segmentation engine generate an automated contour. Since the index case was the only possible match in the atlas, the algorithm selected the index case as the best match. The pre-contoured CT image is then deformably registered onto the patient's empty CT image. The atlas pre-contoured CTV and the five OARs were warped and transformed onto the CT to create a tailored automated contour. The elapsed time for these first three steps was recorded. The auto-contour for the prostate bed, bladder, left and right femoral head, penile bulb and rectum was edited by the atlas builder according to the RTOG guidelines. The time required to edit the CTV and each of the five OARs was recorded. The final contours were then added to the atlas database, totaling two atlas subjects. The atlas builder repeated these steps for the remaining 73 patients that were selected at random. Once the atlas was completed, a second investigator (AVL) audited the final contours to ensure all contours complied with the RTOG consensus guidelines for the delineation of the prostate bed. Thus at the completion of stage I, the AABS engine had 75 reference cases with RTOG compliant segmentation for the generation of automated contours in stages II and III.
where V1 and V2 represent the volumes of the first and the second contours respectively and ∩ is the intersection. As the DSC in contouring studies generally do not follow a normal distribution, a logit transformation was performed to allow for appropriate statistical inferences.
Statistical Analysis Stage One: Atlas Building
Descriptive statistics and Pearson correlation coefficients were calculated to explore the performance and efficiency of the AABS tool (DSC and contour generation time as a function of number of patients in the atlas). The calculated DSC compared the initial, unedited-AABS to the version edited by the atlas builder to gain insights on the performance of the AABS. One-way quintile ANOVA assessed the contouring time in relation to the number of patients in the atlas. Shapiro-Wilk test for normal distribution was performed on the calculated DSC. Quintile ANOVA using logit(DSC) assessed the performance of the AABS engine to generate RTOG compliant segmentations for every 15 patients added to the atlas. Bonferroni correction was used to adjust for multiplicity in the quintile comparisons.
Statistical Analysis Stage Two: Assessment of the AABS and Inter-observer Variability
The DSC was calculated to compare the AABS, expert panel members ("observers") and the atlas builder. Descriptive statistics were calculated to illustrate overall inter-observer variability. Shapiro-Wilk test for normal distribution was performed on the calculated DSC. One-way analysis of variance was performed using logit(DSC) to test for inter-observer variability in the delineation of the CTV and five OARs among the expert panel. Two-way analysis of variance was performed modeling the effects of the observer and patient on logit(DSC) values for the CTV and five OARs, and the effects of the observer and patient on the contouring time.
Statistical Analysis Stage Three: Validation of the Automated Atlas-Based Segmentations
The DSC was calculated for a number of spatial overlap comparisons to determine the convergence of the edited automated contours towards the gold standard and intra-observer variability in the delineation of the CTV and five OARs. Descriptive statistics was calculated to describe the performance of the AABS engine using DSC and the total contouring time for human observers and non-human raters. A paired t-test was performed to assess differences in the time required to edit the auto-contours and the de novo manual contouring time.
Attempts to Minimize Bias
Four measures were taken to minimize bias. The first attempt to eliminate bias occurred at the construction of the RTOG atlas stage through the appraisal of the edited contours by a second radiation oncology expert to ensure compliance. Calculating the DSC between the atlas builder and STAPLE generated consensus contours at stage two to evaluate the appropriateness of that particular radiation oncologist as the atlas builder was the second attempt to minimize bias. Sending the expert panel the anonymized blinded dataset to be assessed in stage three was used as another attempt to minimize bias. In stage three the expert panel was blinded as to the source of the contours in assessing intra-observer variability to hopefully prevent any bias the expert panel may have had if they knew the creator of the contour. Finally, waiting four weeks after the expert panel finished stage two before sending the anonymized data set to the expert panel to be reviewed was designed to prevent the members from recalling their own contours.
In stage one, generating AABS for the 75 patients took an average of 108 seconds per patient (standard deviation, SD = 25 seconds, range 68 to 200 seconds). ANOVA suggested no improvements in auto-contouring time as the number of subjects increased in the atlas (p value = 0.28). The mean (SD) for the auto-contouring time for quintile 1, 2, 3, 4, and 5 were 103 (37), 97 (11), 109 (27), 114 (23) and 115 (22) seconds, respectively (p = 0.282 between quintiles).
The ability of the automated atlas-based segmentation tool to generate segmentations compliant with the consensus guidelines as more subjects are added to the atlas
DSC mean (SD)
DSCs of the CTV and ROIs, assessing auto-contours and inter-observer variability
AC vs. STAPLE
Edited AC vs. STAPLE
AC vs. Expert Panel
STAPLE vs. Expert Panel
Observers vs. Other Observers
AB vs. STAPLE
0.48 (0.17, 0.18-0.59)
0.67 (0.19, 0.18-0.91)
0.47 (0.16, 0.11-0.64)
0.78 (0.15, 0.37-0.91)
0.65 (0.14, 0.29-0.84)
0.93 (0.03, 0.90-0.96)
0.67 (0.19, 0.34-0.80)
0.88 (0.13, 0.34-0.97)
0.67 (0.18, 0.33-0.81)
0.96 (0.02, 0.92-0.98)
0.94 (0.03, 0.87-0.97)
0.97 (0.01, 0.95-0.99)
Left Femoral Head
0.92 (0.01, 0.92-0.93)
0.93 (0.01, 0.92-0.97)
0.83 (0.18, 0.43-0.93)
0.87 (0.19, 0.47-0.98)
0.76 (0.23, 0.42-0.99)
0.96 (0.01, 0.95-0.98)
Right Femoral Head
0.92 (0.01, 0.91-0.93)
0.92 (0.01, 0.90-0.96)
0.83 (0.17, 0.45-0.94)
0.87 (0.19, 0.46-0.98)
0.77 (0.23, 0.46-0.99)
0.97 (0.01, 0.95-0.98)
0.33 (0.25, 0.10-0.70)
0.54 (0.21, 0.10-0.78)
0.31 (0.23, 0-0.78)
0.70 (0.17, 0-0.88)
0.55 (0.22, 0-0.84)
0.84 (0.07, 0.75-0.94)
0.59 (0.11, 0.48-0.77)
0.78 (0.12, 0.49-0.90)
0.58 (0.09, 0.45-0.77)
0.89 (0.06, 0.67-0.94)
0.83 (0.07, 0.65-0.91)
0.94 (0.02, 0.92-0.96)
The spatial overlap between the atlas builder and STAPLE was calculated to determine if the atlas builder contours the CTV and ROI as the community of radiation oncologists would contour these regions. The mean DSC (SD, range) was 0.93 (0.03, 0.90-0.96) for the prostate bed, 0.97 (0.01, 0.95-0.99) for the bladder, 0.96 (0.01, 0.95-0.98) for the left femoral head, 0.97 (0.01, 0.95-0.98) for the right femoral head, 0.84 (0.07, 0.75-0.94) for the penile bulb and 0.94 (0.02, 0.92-0.96) for the rectum.
One-way ANOVA on DSC between Radiation Oncologists was performed to evaluate inter-observer variability. At least one observer significantly differed from the other observers when contouring the prostate bed (p value = 0.002), left femoral head (p value < 0.001) and right femoral head (p value < 0.001). There was no significant difference among observers when contouring the bladder, penile bulb and the rectum. Two-way ANOVA modeling the effects of the observer and patient on the DSC was performed. This revealed significant differences in the delineation of the prostate bed, (p < 0.001). Observer and patient differences significantly predicted for variability in DSC for prostate bed (p < 0.001, p = 0.006) and bladder (p = 0.002, p < 0.001). Variability in right and left femoral heads DSC was significantly dependent on the observer only (both p < 0.001), while variability in rectum and penile bulb delineation was dependent on patient factors (p < 0.001). Another two-way ANOVA analysis modeling the effects of the observers and patients on the contouring time was performed. The full two-way model for the contouring time was significant (p < 0.001) with both the observers (p < 0.001) and the patients (p < 0.001) having a significant effect on the contouring time.
With regards to stage three, Table 2 displays the results of the DSC comparing the edited-auto-contours by the expert panel to the STAPLE. The highest spatial overlap was seen in the left femoral head and the right femoral head, while the lowest spatial overlap was seen in the penile bulb. The second lowest spatial overlap was seen in the prostate bed. These results are consistent with those seen in stage two that compared the unedited auto-contours to STAPLE.
The expert panel was sent an anonymized representative contour sets generated by another expert panel member, the AABS, the STAPLE algorithm. Prior to any editing of the stage two contours by the observers, the observers were asked if the contours were acceptable. Of the 100 cases distributed, 78% of the human contours, 96% of the STAPLE contours, and 12% of the MIM auto-contours were considered clinically acceptable. The expert panel was also asked to identify the source of the contours. Out of the 50 non-human contours, 54% were correctly identified while out of the 50 human contours, 70% was correctly identified. The probability that a Radiation Oncologist was able to properly identify his own contours was 56%.
The panel members were asked to edit the contours as clinically necessary. There appeared to be little intra-observer variability among the edited contours among the expert panel. The penile bulb had the lowest mean DSC at 0.89 (0.04, 0.84-0.98) which is still considered to be good spatial overlap. The remaining OARs intra-observer variability DSC were: prostate bed 0.94 (0.04, 0.84-0.98), bladder 0.98 (0.01, 0.96-0.99), left femoral head 0.97 (0.01, 0.96-0.99), right femoral head 0.97 (0.01, 0.95-0.99), and rectum 0.94 (0.04, 0.80-0.98).
The mean (SD) contouring time for all five cases for the edited auto-contouring time and the manual contouring time was 14.1 minutes (8.4 minutes) and 17.5 minutes (5.4 minutes) respectively, equating to an average 24% time reduction when using the AABS tool. A paired t-test comparing the times of the edited auto-contouring to the manual contouring time showed significant difference in contouring times (p value = 0.003).
Inter-observer variability in segmentation (targets and organs at risk) may be the most significant contributor to uncertainty in radiation treatment planning . We have shown that even with the use of consensus guidelines, inter-observer variability still exists. With these findings, it is important to continue to address the variability challenges. Computerized contouring aids can potentially reduce this variability and increase efficiency in the segmentation workflow and AABS is one such tool. This was the first study to evaluate automated atlas based segmentations for the prostate bed. In this study we evaluated and validated contours created by atlas-based segmentation engines in the context of segmentation of post-prostatectomy radiotherapy planning CT datasets. In the context of this study, only 12% of the unedited contours generated by the AABS were found to be clinically acceptable by the expert panel. Specifically, while the AABS tool appears to reasonably delineate the femoral heads, bladder and rectum, the delineation of the prostate bed and penile bulb were unacceptable. The edited-auto-contours for the femoral heads, bladder and rectum had superior to good spatial overlap when compared to the gold standard. However, the edited-auto-contours for the prostate bed and the penile bulb require improvement when compared to the gold standard. The penile bulb represents a small volume, and thus small variations in its contouring will result in a large change in DSC. In terms of the prostate bed our findings are not surprising given that AABS algorithms are typically developed to detect and segment intact structures and the prostate bed is a "virtual" target defined by boundaries of surrounding normal tissues based on known patterns of recurrence and expert opinion rather than a discrete structure.
The inherent difficulty in defining the "virtual" prostate bed target is reflected in the presence of inter-observer variability in the delineation of the prostate bed and has been repeatedly demonstrated in the literature [2–4, 12, 13]. This variability appears to persist even despite the use of rigorous contouring protocols and guidelines [12, 14–16]. Symon et al., in their study of prostate bed contouring variability, defined a high-risk volume, which on average is missed in 27.5% (range, 2.3%-78.7%) of cases. At least 25% of the high-risk volume at the bladder neck anastomosis and the retro-vesical space was excluded in 11 out of 38 CTVs . Our study found that intra-observer variability was a smaller source of TVD error than inter-observer variability, consistent with the literature [3, 17]. Wiltshire et al. quantified TVD variability using a distance-based approach, and found consistent inter-observer variability within the anterioposterior and superioinferior dimensions measuring a mean (SD) distance between contours of 3.8 mm (2.2 mm) and 1.2 mm (2.3 mm) respectively. The main source of the intra-observer variability in this study was in the anterior-posterior dimension measuring a mean (SD) distance between contours of 0.4 mm (1.2 mm).
The use of AABS tools to delineate OARs for other cancer disease sites including head and neck , breast , and endometrium  have been shown to reduce TVD variability and the total time required to contour; in our study the main benefit of the AABS was in decreasing the amount of time for contouring through editing of the auto contours rather than requiring de novo generation of contours.
The conclusions of this study need to be considered in the context of its limitations. The 80 post-prostatectomy cases used from our institution may limit the applicability of the atlas to other practice groups. Incorporating all available patients into the atlas building process does have a drawback. While increasing the number of patients added to the atlas increases the potential to account for differences in anatomy post surgery, it is at the cost of computational time. The larger the atlas, the longer it will take the tool to search through the atlas to select the best match. Other studies used 10 patients  and one study that assessed the same AABS tool included 15 patients in their atlas . We found no improvement in performance of the AABS when analyzed by quintile; suggesting a dataset of 15 patients may be sufficient to provide auto contours that are useful for subsequent editing/refinement.
This study's methodology builds on the available literature to improve the methodological strength. The strengths of the methodology include the use of consensus guidelines, anonymized datasets, the blinding of observers, the creation of a ground truth, and our specific measures to limit bias, especially with the comparison of the atlas builder to the ground truth. Except for our attempts to limit bias, this methodology is similar to that used in another study . The differences are in the attempts to limit bias and the statistical analyses.
We recommend that the MIM AABS tool can be adopted for routine clinical use to generate auto-contours for the bilateral femoral heads with no editing required. For the bladder and rectum, the auto-contours require some editing by a Radiation Oncologist. Clinical use of the atlas requires a Radiation Oncologist to review and edit the auto-contours, in particular for OARs where the AABS underperforms such as the penile bulb and prostate bed CTV. The automated contouring workflow from a clinical perspective was shown to be significantly shorter than the manual contouring process. The methodology highlights the strengths and areas of improvement for AABS and systematically assesses the presence and amount of inter- and intra-observer variability. If contouring practices for CTVs and OARs converge with the adoption of contouring guidelines, AABS algorithms may be programmed in parallel with these guidelines to optimize how Radiation Oncologists delineate targets. Performing these tasks in a systematic manner through technological assessment as demonstrated in this paper is crucial to ensure the appropriate use of such tools in clinical practice. As the field of AABS advances, it becomes increasingly important to evaluate the accuracy and reliability of the atlas-based segmentations to garner empirical evidence to support the decision-making process prior to its adoption for routine clinical use.
- Bolla M, Collette L: pT3N0M0 prostate cancer: a plea for adjuvant radiation. Nat Rev Urol. 2009, 6 (8): 410-412. 10.1038/nrurol.2009.103.PubMedGoogle Scholar
- Michalski JM, Lawton C, El Naqa I, Ritter M, O'Meara E, Seider MJ, Lee WR, Rosenthal SA, Pisansky T, Catton C, et al: Development of RTOG consensus guidelines for the definition of the clinical target volume for postoperative conformal radiation therapy for prostate cancer. Int J Radiat Oncol Biol Phys. 2010, 76 (2): 361-368. 10.1016/j.ijrobp.2009.02.006.PubMed CentralPubMedGoogle Scholar
- Wiltshire KL, Brock KK, Haider MA, Zwahlen D, Kong V, Chan E, Moseley J, Bayley A, Catton C, Chung PW, et al: Anatomic boundaries of the clinical target volume (prostate bed) after radical prostatectomy. Int J Radiat Oncol Biol Phys. 2007, 69 (4): 1090-1099. 10.1016/j.ijrobp.2007.04.068.PubMedGoogle Scholar
- Mitchell DM, Perry L, Smith S, Elliott T, Wylie JP, Cowan RA, Livsey JE, Logue JP: Assessing the effect of a contouring protocol on postprostatectomy radiotherapy clinical target volumes and interphysician variation. Int J Radiat Oncol Biol Phys. 2009, 75 (4): 990-993. 10.1016/j.ijrobp.2008.12.042.PubMedGoogle Scholar
- Lawton CA, Michalski J, El-Naqa I, Kuban D, Lee WR, Rosenthal SA, Zietman A, Sandler H, Shipley W, Ritter M, et al: Variation in the definition of clinical target volumes for pelvic nodal conformal radiation therapy for prostate cancer. Int J Radiat Oncol Biol Phys. 2009, 74 (2): 377-382. 10.1016/j.ijrobp.2008.08.003.PubMed CentralPubMedGoogle Scholar
- Lawton CA, Michalski J, El-Naqa I, Buyyounouski MK, Lee WR, Menard C, O'Meara E, Rosenthal SA, Ritter M, Seider M: RTOG GU Radiation oncology specialists reach consensus on pelvic lymph node volumes for high-risk prostate cancer. Int J Radiat Oncol Biol Phys. 2009, 74 (2): 383-387. 10.1016/j.ijrobp.2008.08.002.PubMed CentralPubMedGoogle Scholar
- Livsey JE, Wylie JP, Swindell R, Khoo VS, Cowan RA, Logue JP: Do differences in target volume definition in prostate cancer lead to clinically relevant differences in normal tissue toxicity?. Int J Radiat Oncol Biol Phys. 2004, 60 (4): 1076-1081. 10.1016/j.ijrobp.2004.05.005.PubMedGoogle Scholar
- Jameson MG, Holloway LC, Vial PJ, Vinod SK, Metcalfe PE: A review of methods of analysis in contouring studies for radiation oncology. J Med Imaging Radiat Oncol. 2010, 54 (5): 401-410. 10.1111/j.1754-9485.2010.02192.x.PubMedGoogle Scholar
- Zietman A, Goitein M, Tepper JE: Technology evolution: is it survival of the fittest?. J Clin Oncol. 2010, 28 (27): 4275-4279. 10.1200/JCO.2010.29.4645.PubMedGoogle Scholar
- Piper JW: Evaluation of An Intensity-Based Free-form Deformable Registration Algorithm. Medical Physics. 2007, 34 (6): 2353-2354.Google Scholar
- Warfield SK, Zou KH, Wells WM: Simultaneous truth and performance level estimation (STAPLE): an algorithm for the validation of image segmentation. IEEE Trans Med Imaging. 2004, 23 (7): 903-921. 10.1109/TMI.2004.828354.PubMed CentralPubMedGoogle Scholar
- Ost P, De Meerleer G, Vercauteren T, De Gersem W, Veldeman L, Vandecasteele K, Fonteyne V, Villeirs G: Delineation of the Postprostatectomy Prostate Bed Using Computed Tomography: Interobserver Variability Following the EORTC Delineation Guidelines. Int J Radiat Oncol Biol Phys. 2011Google Scholar
- Symon Z, Tsvang L, Wygoda M, Ben-Yoseph R, Corn BW, Poortmans P, Portnoy O, Pfeffer MR: An interobserver study of prostatic fossa clinical target volume delineation in clinical practice: are regions of recurrence adequately targeted?. Am J Clin Oncol. 2011, 34 (2): 145-149.PubMedGoogle Scholar
- Miralbell R, Vees H, Lozano J, Khan H, Molla M, Hidalgo A, Linero D, Rouzaud M: Endorectal MRI assessment of local relapse after surgery for prostate cancer: A model to define treatment field guidelines for adjuvant radiotherapy in patients at high risk for local failure. Int J Radiat Oncol Biol Phys. 2007, 67 (2): 356-361. 10.1016/j.ijrobp.2006.08.079.PubMedGoogle Scholar
- Poortmans P, Bossi A, Vandeputte K, Bosset M, Miralbell R, Maingon P, Boehmer D, Budiharto T, Symon Z, van den Bergh AC, et al: Guidelines for target volume definition in post-operative radiotherapy for prostate cancer, on behalf of the EORTC Radiation Oncology Group. Radiother Oncol. 2007, 84 (2): 121-127. 10.1016/j.radonc.2007.07.017.PubMedGoogle Scholar
- Sidhom MA, Kneebone AB, Lehman M, Wiltshire KL, Millar JL, Mukherjee RK, Shakespeare TP, Tai KH: Post-prostatectomy radiation therapy: consensus guidelines of the Australian and New Zealand Radiation Oncology Genito-Urinary Group. Radiother Oncol. 2008, 88 (1): 10-19. 10.1016/j.radonc.2008.05.006.PubMedGoogle Scholar
- Louie AV, Rodrigues G, Olsthoorn J, Palma D, Yu E, Yaremko B, Ahmad B, Aivas I, Gaede S: Inter-observer and intra-observer reliability for lung cancer target volume delineation in the 4D-CT era. Radiother Oncol. 2010, 95 (2): 166-171. 10.1016/j.radonc.2009.12.028.PubMedGoogle Scholar
- Reed VK, Woodward WA, Zhang L, Strom EA, Perkins GH, Tereffe W, Oh JL, Yu TK, Bedrosian I, Whitman GJ, et al: Automatic segmentation of whole breast using atlas approach and deformable image registration. Int J Radiat Oncol Biol Phys. 2009, 73 (5): 1493-1500. 10.1016/j.ijrobp.2008.07.001.PubMed CentralPubMedGoogle Scholar
- Young AV, Wortham A, Wernick I, Evans A, Ennis RD: Atlas-based segmentation improves consistency and decreases time required for contouring postoperative endometrial cancer nodal volumes. Int J Radiat Oncol Biol Phys. 2011, 79 (3): 943-947. 10.1016/j.ijrobp.2010.04.063.PubMedGoogle Scholar
- Stapleford LJ, Lawson JD, Perkins C, Edelman S, Davis L, McDonald MW, Waller A, Schreibmann E, Fox T: Evaluation of automatic atlas-based lymph node segmentation for head-and-neck cancer. Int J Radiat Oncol Biol Phys. 2010, 77 (3): 959-966. 10.1016/j.ijrobp.2009.09.023.PubMedGoogle Scholar
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