γ-H2AX evaluation post-irradiation. (A) U251 cells were plated onto chamber slides, irradiated at the specific doses, and fixed for immunocytochemical analysis. Foci were evaluated three times in 30 nuclei per treatment per experiment. (B) Plot of the linear dynamic range of the immunofluorescent staining assay. (C) Plot of the linear dynamic range of the MSD Assay.