Resistance of slow proliferating cells to DCQ. A. After plating SCp2 cells in growth media (GM), they were subjected to different conditions. Cells were either kept in GM, or shifted 12 h after plating to differentiation media (DM) or DM+BM (basement membrane) for a more differentiated state. SCp2 cells were incubated for one day then assayed for proliferation using Cell Titer 96 non-radioactive cell proliferation. The proliferation rate of cells grown in GM was considered as 100%. B. The viability of SCp2 cells at different differentiating states were measured by Cytotox 96 non-radioactive cytotoxicity assay directly after 4 h of DCQ treatment.