Interstitial high-dose rate brachytherapy as boost for anal canal cancer
© Falk et al.; licensee BioMed Central Ltd. 2014
Received: 15 February 2014
Accepted: 16 October 2014
Published: 6 November 2014
To assess clinical outcomes of patients treated with a high-dose rate brachytherapy boost for anal canal cancer (ACC).
From August 2005 to February 2013, 28 patients presenting an ACC treated by split-course external beam radiotherapy (EBRT) and HDR brachytherapy with or without chemotherapy in a French regional cancer center in Nice were retrospectively analyzed.
Median age was 60.6 years [34 - 83], 25 patients presented a squamous cell carcinoma and 3 an adenocarcinoma; 21 received chemotherapy. Median dose of EBRT was 45 Gy [43.2 - 52]. Median dose of HDR brachytherapy was 12 Gy [10 - 15] with a median duration of 2 days. Median overall treatment time was 63 days and median delay between EBRT and brachytherapy was 20 days. Two-year local relapse free, metastatic free, disease free and overall survivals were 83%, 81.9%, 71.8% and 87.7% respectively. Acute toxicities were frequent but not severe with mostly grade 1 toxicities: 37% of genito-urinary, 40.7% of gastro-intestinal and 3.7% of cutaneous toxicities. Late toxicities were mainly G1 (43.1%) and G2 (22%). Two-year colostomy-free survival was 75.1%, one patient had a definitive sphincter amputation.
High-dose rate brachytherapy for anal canal carcinoma as boost represents a feasible technique compared to low or pulsed-dose rate brachytherapy. This technique remains an excellent approach to precisely boost the tumor in reducing the overall treatment time.
KeywordsBrachytherapy Anal canal cancer High-dose rate Boost Radiotherapy
Anal canal carcinoma (ACC) is a rare disease with an incidence rate of less than 10 cases per 1 000 000 habitants in Europe , which has been considered as a life deteriorating even when cured because of sphincter amputation caused by historical surgical treatment. The impact on quality of life has pushed clinicians to consider new treatment ways. Conservative treatments for sphincter preservation in ACC have become a standard, even for large tumors. Gold standard treatment consists of concomitant radiotherapy and chemotherapy with Mitomycin and 5-Fluorouracil -. Clinical target volume consists of the gross tumor volume and the loco-regional lymph nodes with a dose of 45 to 50 Gy with a sequential boost delivered by either external beam radiation therapy (EBRT) or by interstitial brachytherapy (BCT) ,. All attempts to find a better standard of chemotherapy have failed . However, recent development of radiotherapy technique have permitted better care and new treatment strategies for ACC ,.
There is no agreement for the boost technique. Standard brachytherapy technique remains based on LDR or PDR ,. However as proposed for numerous tumors (prostate  and cervical cancer ) HDR brachytherapy appears to be more and more used mainly because of radioprotection considerations, dose distribution optimization and shorter treatment time . Currently, there is few available data focusing on high-dose rate brachytherapy for ACC. This study was aimed to assess clinical outcomes of patients treated with a HDR brachytherapy boost for anal canal cancer.
Material and methods
This study retrospectively analyzed the clinical outcome of patients with histology-proven ACC treated by split-course radiotherapy and HDR brachytherapy with or without chemotherapy in the Centre Antoine Lacassagne, a regional cancer center in Nice, France. The study design and analysis was approved by the local institutional ethic comity of Antoine Lacassagne Cancer Center.
From August 2005 to February 2013, 28 patients were retrospectively analyzed. All the patients had a clinical exam and follow-up done by trained physicians of the center. According to local rules, patients underwent a digital rectal examination with a dated schema, anuscopy, computed-tomography scan, magnetic resonance imaging and endorectal ultrasonography. PET-scan was performed at the discretion of the physician. Tumors were staged using the UICC-cTNM classification (2002 - 6th edition). All the patients presenting with a tumor involving no more than 2/3 of the anal canal circumference were eligible for brachytherapy boost. Due to a high risk of necrosis/stenosis, circumferential tumor was considered as a definitive exclusion criteria for brachytherapy.
The first part of the treatment consisted of EBRT with or without concurrent chemotherapy. Radiotherapy delivered a total dose of 45 to 46 Gy in 25 or 23 fractions based on a 3-dimensional conformational technique with or without intensity modulated. The dose was delivered to the ICRU point (International Commission on Radiation Units Measurements) using high-energy X-photons (>10 MV). The clinical target volume (CTV) was the whole pelvis. The planning target volume (PTV) was defined as a 1 cm margin around the CTV in all directions. Inclusion of the inguinal nodes was set at the discretion of the radiation oncologist.
Patients who received concomitant chemotherapy were administrated Mitomycin C - 5FU or Cisplatin - 5FU.
HDR brachytherapy boost
Brachytherapy boost was performed unless patients could not undergo general anesthesia or presented with a tumor invasion >2/3 of the circumference. HDR brachytherapy was performed after the patient recovered from perineal dermatitis. After a 2 day fiber free diet and enema the day before and one-hour before procedure. A digital exam and anuscopy were performed under general anesthesia in order to evaluate the tumor response after radio-chemotherapy. A Foley catheter was first introduced into the bladder, then, needles (Sharp Needles™; Nucletron, an Elekta company, Elekta AB, Stockholm, Sweden) were implanted under general anesthesia according to the pre-treatment target volume (based on the initial schema) respecting a minimal distance of 4 to 5 mm from the needles to the anal canal mucosa. We used a dedicated circular perineal template punched by a total of 10 holes (every 12 mm) allowing to keep the implanted needles equidistant and parallel. A plastic tube (20 mm in external diameter) was placed into the anal canal and fixed to the perineal template which was finally sutured to the skin. After recovery, post-implant planning CT-scan was performed in the radiation oncology department for treatment planning purposes. CTV was delineated (anal canal initial tumor) using a 10 mm in diameter pearl.
Regarding dosimetric results, D90 (dose delivered to 90% of the CTV) and D100 were reported as well as V100 (volume receiving 100% of the prescribed dose), V150 and V200. DHI (Dose Homogeneity Index: [V100-V150]/V100) was also calculated.
Follow-up and evaluation
Patients were followed-up one month after HDR brachytherapy then, every semester with radiation oncologist and gastro-enterologist alternatively by clinical examination, endorectal ultrasonography and MRI if necessary. Local (LRFS) and metastatic (MRFS) relapse-free survivals as well as disease free (DFS), overall (OS) and colostomy-free (CFS) survivals were analyzed. Early and late toxicities rates were graded using the NCI-Common criteria version 4.0 .
All the data was analyzed using SPSS 20.0 (IBM Corporation). Overall survival was defined as the delay between date of diagnostic and the date of death. Relapse was defined as the delay between the date of diagnostic and the date of relapse. These statistics were estimated and represented graphically using Kaplan-Meier method. Patients were censored at the moment of their death or their last follow-up.
Patient, tumor and treatment features
[34 - 83]
Sex ratio (F/M)
Lymph node status
Total dose (Gy)
[43.2 - 52]
[3 - 5]
# of fractions
[2 - 6]
Total dose (Gy)
[10 - 15]
# of needles
[4 - 7]
TI EBRT/BT (days)
[4 - 63]
[38 - 74]
[8.6 - 46.7]
[32 - 117]
EQD2 αβ10 (Gy)
[5.5 - 20.6]
EQD2 αβ3 (Gy)
[6.9 - 26.4]
[20 - 95]
EQD2 αβ10 (Gy)
[3.4 - 14.4]
EQD2 αβ3 (Gy)
[4.3 - 18.5]
[58 - 100]
[8.1 - 45.8]
[26 - 57]
[2.5 - 26.6]
[10 - 26]
[1.2 - 11.7]
[0.42 - 0.99]
Brachytherapy plays a key role in the management of ACC as boost after a first course of EBRT with or without concomitant chemotherapy . From now, brachytherapy for ACC used mainly LDR or PDR. However, due to the possibilities of dose distribution optimization, but also radioprotection and cost-effective considerations, HDR brachytherapy gains, step by step, in respectability. While this technique represents a standard treatment for cervical  and prostate cancers , there is very few available data concerning ACC.
Optimization of dose distribution used with PDR/HDR brachytherapy represents in general a real advantage compare to LDR brachytherapy. However, specifically for ACC brachytherapy, because of the very proximal situation of the CTV in regards to the perineal template used (optimal needle guidance), the impact of dose optimization is maybe less important although adequate coverage of the anal mucosa (avoiding overdose area) appears more feasible. Classical dosimetric parameters used for HDR brachytherapy (CTV, D90, D10, V100, V150 and V200) can be used for ACC. Kapoor et al.  recently reported the results of 16 patients treated by radio-chemotherapy and HDR brachytherapy and presented dosimetric data with a mean DHI of 0.83 [0.55-0.98]. In our study, we reported a median DHI of 0.58.
Comparative overview of the literature focusing on brachytherapy boost for anal canal carcinoma according to the brachytherapy dose-rate
Median FU (months)
Median BT dose (Gy)
Late toxicity ≥ G3 [%]
Peiffert et al. (1997) 
60 (5 y)
Papillon et al. (1989) 
20 to 30
69.2 (3 y)
Wagner et al. (1994) 
35.5 to 51.7**
64 (5 y)
Gerard et al. (1999) 
10 to 25
100 (1 y)
Bruna et al. (2006) 
89 (2 y)
Roed et al. (1996) 
Kapp et al. (2001) 
6 to 12***
81 (3 y)
80 (3 y DSS)
78 (3 y)
76 (5 y)
76 (5 y DSS)
73 (5 y)
Vordermark et al. (2001) 
5 to 12
84 (5 y)
68.9 (5 y)
Doniec et al. (2006) 
8 to 12
74 (5 y)
Oehler-Jänne et al. (2007) 
66 (10 y)
Saarilahti et al. (2008) 
Kapoor et al. (2013) 
87.5 (2 y)
Falk et al. (2014)
83 (2 y)
75.1 (2 y)
Overview of the literature focusing on external radiotherapy for anal canal carcinoma
Median FU (months)
Median EBRT dose (Gy)
Late toxicity ≥ G3 [%]
Northover et al. (2010) 
57.1 (5 y)
33.7 (5 y)
53 (5 y)
36.8 (5 y)
Northover et al. (2010) 
2DRT + CT
32.3 (5 y)
46.6 (5 y)
58.1 (5 y)
46.9 (5 y)
Ajani et al. (2008) 
25 (5 y)
84% (5 y)
Kachnic et al. (2013) 
95% (2 y)
94% (2 y)
90% (2 y)
Our patients presented frequent grade 1-2 toxicities but grade 3 toxicities only occurred for 7.1% of patients. Severe toxicities (≥ G3) were reported in 0 to 13.5% of patients in LDR/PDR studies ,,,,, and in 0 to 7.6% in HDR studies ,. Only 2 patients presented severe grade 3 toxicities, one of them with an HIV-positive status which could account for higher toxicities . Compared to our results, Kapoor et al.  reported no grade 3 toxicities with a similar LCR. This observation could be explained by the difference in terms of DHI with a less homogeneous implant in our cohort. On another hands, the total number of HDR brachytherapy fractions ranged between 6 and 7 in the Kapoor et al. study while in our cohort, the patients were treated with only 3 fractions (potential reduction of the hospitalization time). Nevertheless, our CFS rate (75.1%) remains comparable to other published data on LDR, PDR and HDR brachytherapy ,.
Due to its design, we weren't able to access quality of life. To our knowledge, there is no comparative assessing quality of life for brachytherapy boost. External radio chemotherapy seems to be associated with good quality of life with 77% patients being satisfied of their body . Recent international guidelines have encouraged the study and evaluation of sexual, urinary and sphincter dysfunction as the data are scarce .
This study presented limitations. Data collection was retrospective and monocentric. Median follow-up was short (27.5 months). We didn't directly compare our data to low or pulsed-dose rate brachytherapy or EBRT boost.
HDR brachytherapy has advantages compared to low or PDR brachytherapy. It permits less radiation exposure for the medical staff and could provide better dose-distribution compared to LDR brachytherapy. Patients can be hospitalized in a non-shielded room allowing visits of the family (forbidden in case of LDR or PDR brachytherapy) with a potential improvement of psychological experience. Furthermore, with only one after-loader HDR machine, is it possible to treat many patients per day while for LDR or PDR, the number of treated patients per day will be strongly correlated to the number of shielded-rooms (potential cost-effective impact). To our knowledge, there are no prospective studies evaluating HDR brachytherapy for anal canal cancer boost after sequential EBRT. Further data needs to be collected and maybe even compared to LDR/PDR brachytherapy.
High-dose rate for anal canal carcinoma as boost represents a feasible technique compared to low or pulsed-dose rate brachytherapy. This technique remains an excellent approach to precisely boost the tumor in reducing the overall treatment time while high-dose rate is more and more used in the frame of brachytherapy. Currently simultaneous-integrated boost (SIB) in case of intensity-modulated EBRT represents a challenging therapeutic option which could be compared to HDR brachytherapy for ACC boost.
Written informed consent was obtained from the patient for the publication of this report and any accompanying images.
AF analyzed the clinical data managed the statistical analysis and wrote the manuscript; AC analyzed the clinical data; KB revised the "results" and "discussion" sections; EF revised the "discussion" section; MG analyzed and revised the dosimetric results; JPG revised the manuscript; JMHL conceived, planned the study and co-wrote the manuscript. All authors read and approved the final manuscript.
- Faivre J, Trama A, De Angelis R, Elferink M, Siesling S, Audisio R, Bosset JF, Cervantes A, Lepage C: Incidence, prevalence and survival of patients with rare epithelial digestive cancers diagnosed in Europe in 1995-2002. Eur J Cancer. 2012, 48: 1417-1424. 10.1016/j.ejca.2011.10.038.View ArticlePubMedGoogle Scholar
- Bartelink H, Roelofsen F, Eschwege F, Rougier P, Bosset JF, Gonzalez DG, Peiffert D, van Glabbeke M, Pierart M: Concomitant radiotherapy and chemotherapy is superior to radiotherapy alone in the treatment of locally advanced anal cancer: results of a phase III randomized trial of the European Organization for Research and Treatment of Cancer Radiotherapy and Gastrointestinal Cooperative Groups. J Clin Oncol Off J Am Soc Clin Oncol. 1997, 15: 2040-2049.Google Scholar
- UKCCCR Anal Cancer Trial Working Party: Epidermoid anal cancer: results from the UKCCCR randomised trial of radiotherapy alone versus radiotherapy, 5-fluorouracil, and mitomycin. UKCCCR Anal Cancer Trial Working Party. UK Co-ordinating Committee on Cancer Research. Lancet. 1996, 348: 1049-1054. 10.1016/S0140-6736(96)03409-5.View ArticleGoogle Scholar
- Epidermoid anal cancer: results from the UKCCCR randomised trial of radiotherapy alone versus radiotherapy, 5-fluorouracil, and mitomycin. Lancet 1996, 348:1049-1054.,Google Scholar
- Northover J, Glynne-Jones R, Sebag-Montefiore D, James R, Meadows H, Wan S, Jitlal M, Ledermann J: Chemoradiation for the treatment of epidermoid anal cancer: 13-year follow-up of the first randomised UKCCCR Anal Cancer Trial (ACT I). Br J Cancer. 2010, 102: 1123-1128. 10.1038/sj.bjc.6605605.PubMed CentralView ArticlePubMedGoogle Scholar
- Myerson RJ, Garofalo MC, Naqa IE, Abrams RA, Apte A, Bosch WR, Das P, Gunderson LL, Hong TS, Kim JJJ, Willett CG, Kachnic LA: Elective clinical target volumes for conformal therapy in anorectal cancer: an RTOG consensus panel contouring atlas. Int J Radiat Oncol Biol Phys. 2009, 74: 824-830. 10.1016/j.ijrobp.2008.08.070.PubMed CentralView ArticlePubMedGoogle Scholar
- Peiffert D, Giovannini M, Ducreux M, Michel P, François E, Lemanski C, Mirabel X, Cvitkovic F, Luporsi E, Conroy T, Gérard J-P: High-dose radiation therapy and neoadjuvant plus concomitant chemotherapy with 5-fluorouracil and cisplatin in patients with locally advanced squamous-cell anal canal cancer: Final results of a phase II study. Ann Oncol. 2001, 12: 397-404. 10.1023/A:1011107105538.View ArticlePubMedGoogle Scholar
- Kachnic LA, Winter K, Myerson RJ, Goodyear MD, Willins J, Esthappan J, Haddock MG, Rotman M, Parikh PJ, Safran H, Willett CG: RTOG 0529: a phase 2 evaluation of dose-painted intensity modulated radiation therapy in combination with 5-fluorouracil and mitomycin-C for the reduction of acute morbidity in carcinoma of the anal canal. Int J Radiat Oncol Biol Phys. 2013, 86: 27-33. 10.1016/j.ijrobp.2012.09.023.PubMed CentralView ArticlePubMedGoogle Scholar
- Papillon J, Montbarbong JF, Gerard JP, Chassard JL, Ardiet JM: Interstitial curietherapy in the conservative treatment of anal and rectal cancers. Int J Radiat Oncol. 1989, 17: 1161-1169. 10.1016/0360-3016(89)90521-X.View ArticleGoogle Scholar
- Bruna A, Gastelblum P, Thomas L, Chapet O, Bollet MA, Ardiet J-M, Gérard J-P, Peiffert D: Treatment of squamous cell anal canal carcinoma (SCACC) with pulsed dose rate brachytherapy: A retrospective study. Radiother Oncol. 2006, 79: 75-79. 10.1016/j.radonc.2006.03.013.View ArticlePubMedGoogle Scholar
- Hoskin PJ, Colombo A, Henry A, Niehoff P, Paulsen Hellebust T, Siebert F-A, Kovacs G: GEC/ESTRO recommendations on high dose rate afterloading brachytherapy for localised prostate cancer: an update. Radiother Oncol J Eur Soc Ther Radiol Oncol. 2013, 107: 325-332. 10.1016/j.radonc.2013.05.002.View ArticleGoogle Scholar
- Viswanathan AN, Thomadsen B, American Brachytherapy Society Cervical Cancer Recommendations Committee, American Brachytherapy Society: American Brachytherapy Society consensus guidelines for locally advanced carcinoma of the cervix. Part I: general principles. Brachytherapy. 2012, 11: 33-46. 10.1016/j.brachy.2011.07.003.View ArticlePubMedGoogle Scholar
- Kolotas C, Zamboglou N: Role of interstitial brachytherapy in the treatment of malignant disease. Onkologie. 2001, 24: 222-228. 10.1159/000055084.View ArticlePubMedGoogle Scholar
- NCI Term Browser. [http://nciterms.nci.nih.gov/ncitbrowser/pages/vocabulary.jsf?dictionary=Common%20Terminology%20Criteria%20for%20Adverse%20Events]
- Kapoor R, Khosla D, Shukla AK, Kumar R, Gupta R, Oinam AS, Sharma SC: Dosimetric and clinical outcome in image-based high-dose-rate interstitial brachytherapy for anal cancer. Brachytherapy 2013. no 4 (July 2014): 388-93. doi:10.1016/j.brachy.2013.09.006.,Google Scholar
- Vordermark D, Flentje M, Sailer M, Kölbl O: Intracavitary afterloading boost in anal canal carcinoma. Results, function and quality of life. Strahlenther Onkol Organ Dtsch Röntgenges Al. 2001, 177: 252-258. 10.1007/PL00002405.View ArticleGoogle Scholar
- Doniec JM, Schniewind B, Kovacs G, Kahlke V, Loehnert M, Kremer B: Multimodal therapy of anal cancer added by new endosonographic-guided brachytherapy. Surg Endosc. 2006, 20: 673-678. 10.1007/s00464-004-2287-z.View ArticlePubMedGoogle Scholar
- Oehler-Jänne C, Seifert B, Lütolf UM, Studer G, Glanzmann C, Ciernik IF: Clinical outcome after treatment with a brachytherapy boost versus external beam boost for anal carcinoma. Brachytherapy. 2007, 6: 218-226. 10.1016/j.brachy.2007.02.152.View ArticlePubMedGoogle Scholar
- Saarilahti K, Arponen P, Vaalavirta L, Tenhunen M: The effect of intensity-modulated radiotherapy and high dose rate brachytherapy on acute and late radiotherapy-related adverse events following chemoradiotherapy of anal cancer. Radiother Oncol. 2008, 87: 383-390. 10.1016/j.radonc.2008.04.011.View ArticlePubMedGoogle Scholar
- Gerard J-P, Mauro F, Thomas L, Castelain B, Mazeron J-J, Ardiet J-M, Peiffert D: Treatment of squamous cell anal canal carcinoma with pulsed dose rate brachytherapy. Feasibility study of a French cooperative group. Radiother Oncol. 1999, 51: 129-131. 10.1016/S0167-8140(99)00049-3.View ArticlePubMedGoogle Scholar
- López Guerra JL, Lozano AJ, Pera J, Gutiérrez C, Cambray M, Ferrer F, Guedea F: Twenty-year experience in the management of squamous cell anal canal carcinoma with interstitial brachytherapy. Clin Transl Oncol Off Publ Fed Span Oncol Soc Natl Cancer Inst Mex. 2011, 13: 472-479.Google Scholar
- Ajani, Jaffer A., Kathryn A. Winter, Leonard L. Gunderson, John Pedersen, Al B. Benson, Charles R. Thomas, Robert J. Mayer, Michael G. Haddock, Tyvin A. Rich, et Christopher Willett. « Fluorouracil, Mitomycin, and Radiotherapy vs Fluorouracil, Cisplatin, and Radiotherapy for Carcinoma of the Anal Canal: A Randomized Controlled Trial ». JAMA 299, no 16 (April 2008): 1914-21. doi:10.1001/jama.299.16.1914.,Google Scholar
- Kachnic LA, Tsai HK, Coen JJ, Blaszkowsky LS, Hartshorn K, Kwak EL, Willins JD, Ryan DP, Hong TS: Dose-painted intensity-modulated radiation therapy for anal cancer: a multi-institutional report of acute toxicity and response to therapy. Int J Radiat Oncol. 2012, 82: 153-158. 10.1016/j.ijrobp.2010.09.030.View ArticleGoogle Scholar
- Peiffert D, Tournier-Rangeard L, Gérard J-P, Lemanski C, François E, Giovannini M, Cvitkovic F, Mirabel X, Bouché O, Luporsi E, Conroy T, Montoto-Grillot C, Mornex F, Lusinchi A, Hannoun-Lévi J-M, Seitz J-F, Adenis A, Hennequin C, Denis B, Ducreux M: Induction chemotherapy and dose intensification of the radiation boost in locally advanced anal canal carcinoma: final analysis of the randomized UNICANCER ACCORD 03 trial. J Clin Oncol. 2012, 30: 1941-1948. 10.1200/JCO.2011.35.4837.View ArticlePubMedGoogle Scholar
- Wagner J-P, Mahe MA, Romestaing P, Rocher FP, Berger C, Trillet-Lenoir V, Gerard J-P: Radiation therapy in the conservative treatment of carcinoma of the anal canal. Int J Radiat Oncol. 1994, 29: 17-23. 10.1016/0360-3016(94)90221-6.View ArticleGoogle Scholar
- Roed H, Engelholm SA, Svendsen LB, Rosendal F, Olsen KJ: Pulsed dose rate (PDR) brachytherapy of anal carcinoma. Radiother Oncol. 1996, 41: 131-134. 10.1016/S0167-8140(96)01819-1.View ArticlePubMedGoogle Scholar
- Kapp KS, Geyer E, Gebhart FH, Oechs AC, Berger A, Hebenstreit J, Stoeger H: Experience with split-course external beam irradiation ± chemotherapy and integrated Ir-192 high-dose-rate brachytherapy in the treatment of primary carcinomas of the anal canal. Int J Radiat Oncol. 2001, 49: 997-1005. 10.1016/S0360-3016(00)01427-9.View ArticleGoogle Scholar
- Kapp KS, Kapp DS, Stuecklschweiger G, Berger A, Geyer E: Insterstitial hyperthermia and high dose rate brachytherapy in the treatment of anal cancer: A phase I/II study. Int J Radiat Oncol. 1994, 28: 189-199. 10.1016/0360-3016(94)90157-0.View ArticleGoogle Scholar
- Oehler-Jänne C, Huguet F, Provencher S, Seifert B, Negretti L, Riener M-O, Bonet M, Allal AS, Ciernik IF: HIV-specific differences in outcome of squamous cell carcinoma of the anal canal: a multicentric cohort study of HIV-positive patients receiving highly active antiretroviral therapy. J Clin Oncol Off J Am Soc Clin Oncol. 2008, 26: 2550-2557. 10.1200/JCO.2007.15.2348.View ArticleGoogle Scholar
- Provencher S, Oehler C, Lavertu S, Jolicoeur M, Fortin B, Donath D: Quality of life and tumor control after short split-course chemoradiation for anal canal carcinoma. Radiat Oncol. 2010, 5: 41-10.1186/1748-717X-5-41.PubMed CentralView ArticlePubMedGoogle Scholar
- Glynne-Jones R, Nilsson PJ, Aschele C, Goh V, Peiffert D, Cervantes A, Arnold D: Anal cancer: ESMO-ESSO-ESTRO clinical practice guidelines for diagnosis, treatment and follow-up. Radiother Onco. 111, no 3 (June 2014): 330-39. doi:10.1016/j.radonc.2014.04.013.,Google Scholar
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